Swiss Flow
Cytometry School

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Courses

We propose online and on-site courses.
We adapt our offer to your needs : feel free to make suggestions !

Introductory and refresher course in flow cytometry

Working principals of a flow cytometer, set-up and calibration, compensation, fluorescence and fluorochromes, pre-analytical methods, immunodetection, data analysis, strategies for the analysis of peripheral blood samples, applications in immunology.

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Course : INTRO-01
Duration : 30 hours in 5 days (17h lectures, 13h practicals)
Language : English
Target Audience : Anyone working in the biomedical field with no prior knowledge of flow cytometry, users lacking the fundamentals of flow cytometry.
Prerequisites : Basic knowledge in biology and medicine
Courses objectives : The students will learn :
• the basic principles of FCM.
• how to perform multicolor staining experiments with adequate compensations.
• how to elaborate and apply various gating strategies for data analysis.
• the most common applications of flow cytometry in hematology and immunology.
Costs : Public Sector: 2000 CHF
Industry, Pharma: 2500 CHF
Developing Countries: 1200 CHF
Registration deadline for October session: September 15th or when the place limit is reached.

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ONLINE introductory and refresher course in flow cytometry

Interactive meetings in small groups (around 10 participants) with theoretical parts followed by practical exercises and analyses of patient files. Topics: basic principles of flow cytometry, simple multicolor staining, compensations, gating strategies, data analysis, most common applications of flow cytometry in hematology and immunology.

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Course : ONL_INTRO-17
Duration : 6 sessions of 1.5 h over 2 weeks, 1PM-2.30PM (CET)
Language : English
Target Audience : Anyone working in the biomedical field with no prior knowledge of flow cytometry, users lacking the fundamentals of flow cytometry.
Prerequisites : Basic knowledge in biology or medicine.
Courses objectives : Sessions:
1. Introduction to flow cytometry – what is behind the dot plot.
2. Working principles of a flow cytometer: fluidics, optics, electronics.
3. Fluorochromes – how to choose the best one for your application.
4. Immunodetection – antibodies, staining, immunophenotyping.
5. Gating strategies – how to analyze flow cytometry data.
6. Instrument setup and compensation.

The students will learn:
• the basic principles of flow cytometry
• how to perform a simple multicolor staining experiments with adequate compensations.
• how to elaborate and apply various gating strategies for data analysis.
• the most common applications of flow cytometry in hematology and immunology.

Costs : Public Sector: 600 CHF
Industry, Pharma: 800 CHF
Developing Countries: 300 CHF

Dates:
Session I: 23th November – 9th December 2020, Mondays and Wednesdays. Exact dates of each session: 23.11, 25.11, 30.11, 02.12, 07.12, 09.12.

Session II: 18th January – 4th February 2021, Mondays and Thursdays. Exact dates of each session:
18.01 and 21.01 at 1PM-2.30PM (CET)
25.01, 28.01, 01.02 and 04.02 at 8.30AM-10AM (CET)

Session III: 11th – 29th March 2021, Thursdays and Mondays. Exact dates of each session:
11.03, 15.03, 18.03, 22.03, 25.03, 29.03
Time: 2.30-4.00PM (CET)

Session IV: 29th November – 14th December 2021, Mondays and Tuesdays. Exact dates of each session:
29.11, 30.11, 6.12, 7.12, 13.12, 14.12
Time: 11AM-12.30PM (CET)

Session V:
7th - 17th March 2022; Mondays, Tuesdays and Thursdays.
Exact dates of each session: 7.03, 8.03, 10.03, 14.03, 15.03, 17.03.
Time: 1PM-2.30PM (CET)

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Advanced applications of flow cytometry

Multicolor panel design, compensation and setting pitfalls, functional test of apoptosis, cell sorting.

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Course : ADVANCED-04
Duration : 18 hours over 3 days (9h lectures, 9h practicals).
Language : English
Target Audience : Doctoral students, post-docs, researchers.
Prerequisites : Studies in biology, biotechnology, pharmacy, immunology, medicine or related disciplines.
Courses objectives : The participants will learn :
• to design and perform multicolor staining experiments.
• to study cell apoptosis.
• how to avoid compensation and setting pitfalls.
• how to prepare cell sorting experiment.
Costs : Public Sector: 1200 CHF
Industry, Pharma: 1500 CHF
Developing Countries: 800 CHF

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ONLINE advanced applications of flow cytometry

Interactive meetings in small groups (around 10 participants) with theoretical parts followed by practical exercises and analyses of patient files. Topics: compensation and setting pitfalls, multicolor panel, standarization, harmonization, cell cycle, apoptosis, analysis of rare cell populations (including CAR-T cells).

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Course : ONL_ADVANCED-18
Duration : 6 sessions of 1.5 h over 2 weeks, 1PM-2.30PM (CET)
Language : English
Target Audience : Doctoral students, post-docs, researchers.
Prerequisites : Studies in biology, biotechnology, pharmacy, immunology, medicine or related disciplines.
Courses objectives : Sessions:
1. Compensation and setting pitfalls.
2. Multicolor staining.
3. Standardization, QC, harmonization.
4. Cell cycle analysis.
5. Apoptosis, cell proliferation.
6. Analysis of rare events, including CAR-T cells.

The participants will learn:
• to design multicolor panel.
• to standardize and harmonize flow cytometers.
• to study cell cycle and apoptosis.
• how to avoid compensation and setting pitfalls.
• how to analyze rare cell populations, including CAR-T cells.

Costs : Public Sector: 600 CHF
Industry, Pharma: 800 CHF
Developing Countries: 300 CHF

Dates:
7th - 17th March 2022; Mondays, Tuesdays and Thursdays.
Exact dates of each session: 7.03, 8.03, 10.03, 14.03, 15.03, 17.03.
Time: 1PM-2.30PM (CET)

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Flow cytometry for hematologists and immunologists

Multicolor panel design, compensation and setting pitfalls, harmonization and standardization of different flow cytometers, study of cell apoptosis, advanced data analysis, hematopoiesis and analysis of stem cells, immature and mature cell populations, specific applications in hematology, detailed case studies.

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Course : HEMATO_IMMUNO-19
Duration : 22 hours over 3 1/2 days (10h lectures, 12h practical).
Language : English
Target Audience : Hematologists, immunologists, laboratory heads, researchers, advanced biomedical and laboratory technicians, post-docs.
Prerequisites : Knowledge of the basic principles of flow cytometry, strong background in hematology and/or cell biology.
Courses objectives : The participants will learn :
• to design and perform multicolor staining experiments,
• to analyse in depth specific cell populations like stem cells, precursor cells, CAR-T cells,
• to perform standardized set-ups with different cytometers,
• to study cell apoptosis,
• how to avoid compensation and setting pitfalls.
Costs : Public Sector: 1500 CHF
Industry, Pharma: 1800 CHF
Developing Countries: 900 CHF
Registration deadline: November 17th or when the place limit is reached.

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Lymphoma diagnosis and MRD detection by flow cytometry

Normal differentiation of B and T lymphocytes, pathophysiology of lymphomas, differential diagnosis of B and T cell lymphomas (WHO 2016 classification), principles, statistics and practical applications of MRD detection, analysis of MRD in CLL and multiple myeloma, detailed case studies.

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Course : LYMPHOMA_MRD-05
Duration : 22 hours over 3 1/2 days (10h lectures, 12h practical).
Language : English
Target Audience : Researchers with an interest in B and T cell lymphomas, hematologists, immunologists.
Prerequisites : Knowledge of basic principles of flow cytometry, background in hematology and/or cell biology.
Courses objectives : The students will learn :
• how to elaborate and apply gating strategies for lymphocyte analysis.
• how to use cytometry for the detection of normal B and T cells in lymphoid organs, peripheral blood and bone marrow.
• how to diagnose B and T cell lymphomas using cytometry.
• the pitfalls of lymphoma diagnosis and how to avoid them.
• how to perform MRD detection for lymphomas.
Costs : Public Sector: 1500 CHF
Industry, Pharma: 1800 CHF
Developing Countries: 1000 CHF

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ONLINE Lymphoma and MRD course

Interactive meetings in small groups (around 10 participants) with theoretical parts followed by practical exercises and analyses of patient files. Topics: normal differentiation of B and T lymphocytes, pathophysiology of lymphomas, differential diagnosis of B and T cell lymphomas (WHO 2016 classification), principles, statistics and practical applications of MRD detection, analysis of MRD in CLL and multiple myeloma, detailed case studies.

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Course : ONL_LYMPHOMA-16
Duration : 6 sessions of 1.5 hours, twice a week, 1PM-2.30PM (CET)
Language : English
Target Audience : Researchers with an interest in lymphomas, hematologists, immunologists.
Prerequisites : Knowledge of basic principles of flow cytometry, background in hematology and/or cell biology.
Courses objectives : Sessions – the application of the flow cytometry to study:
1. Normal B cell maturation and B cell populations.
2. B cell lymphomas.
3. Normal T cell maturation and T cell populations.
4. T and NK cell lymphomas.
5. Plasma cells – normal and malignant.
6. MRD detection in lymphomas.

The participants will learn the basics of following topics:
• how to elaborate and apply gating strategies for lymphocyte analysis.
• how to use cytometry for the detection of normal B and T cells in lymphoid organs, peripheral blood and bone marrow.
• how to diagnose B and T cell lymphomas using cytometry.
• how to perform MRD detection for lymphomas.

Costs : Public Sector: 600 CHF
Industry, Pharma: 800 CHF
Developing Countries: 300 CHF

Dates:
8th February – 4th March 2021, Mondays and Thursdays.
Exact dates of each session: 8.02, 11.02, 15.02, 18.02, 01.03, 04.03.
Time: 1PM-2.30PM (CET)

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Leukemia diagnosis and MRD detection by flow cytometry

Normal hematopoiesis and its analysis by flow cytometry, analysis of stem cell population, differential diagnosis of acute leukemias using morphology and cytometry (WHO 2016 classification), data analysis, principles, statistics and practical applications of MRD detection; analysis of MRD in acute leukemias, detailed case studies.

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Course : LEUKEMIA_MRD-06
Duration : 22 hours over 3 1/2 days (10h lectures, 12h practical).
Language : English
Target Audience : Researchers with an interest in acute leukemias, hematologists, immunologists.
Prerequisites : Knowledge of basic principles of flow cytometry, background in hematology and/or cell biology.
Courses objectives : The students will learn :
• how to use cytometry for the detailed analysis of normal hematopoiesis in peripheral blood and bone marrow samples.
• how to diagnose acute leukemias (AML and ALL) using cytometry.
• the pitfalls of leukemia diagnosis and how to avoid them.
• how to perform MRD detection in acute leukemias.
Costs : Public Sector: 1500 CHF
Industry, Pharma: 1800 CHF
Developing Countries: 1000 CHF

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ONLINE Leukemia and MRD course

Interactive meetings in small groups (around 10 participants) with theoretical parts followed by practical exercises and analyses of patient files. Topics: normal hematopoiesis and its analysis by flow cytometry, differential diagnosis of acute leukemias using flow cytometry, data analysis, principles, statistics and practical applications of MRD detection; analysis of MRD in acute leukemias, detailed case studies.

course details



Course : ONL_LEUKEMIA-15
Duration : 6 sessions of 1.5 hours, twice a week, 11AM-12.30PM (CET)
Language : English
Target Audience : Researchers with an interest in acute leukemias, hematologists, immunologists.
Prerequisites : Knowledge of basic principles of flow cytometry, background in hematology and/or cell biology.
Courses objectives : Sessions – the application of the flow cytometry to study:
1. Normal hematopoiesis, stem cell compartment.
2. B- and T-ALL (Acute Lymphoblastic Leukemia).
3. AML (Acute Myeloid Leukemia).
4. MDS (Myelodysplastic Syndrome).
5. MRD (Minimal Residual Disease) – theory.
6. MRD in ALL and AML.

The participants will learn the basics of following topics:
• how to use cytometry for the detailed analysis of normal hematopoiesis in peripheral blood and bone marrow samples.
• how to diagnose acute leukemia (AML and ALL) as well as MDS using cytometry.
• how to perform MRD detection in acute leukemia.

Costs : Public Sector: 600 CHF
Industry, Pharma: 800 CHF
Developing Countries: 300 CHF

Dates:
January 17th - 27th, 2023
Exact dates of each session: 17.01, 19.01, 20.01, 24.01, 26.01, 27.01.
Time: 11AM-12.30PM (CET)

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Introduction to modern computational cytometry techniques

The aim of this workshop is to introduce the participants to modern computational cytometry techniques. A mixture of theoretical and practical courses will show the participants how to set up a pipeline using the R programming language, including key steps such as data preprocessing and transformation, automated identification of cell populations with the FlowSOM algorithm and differential analysis of sample groups. No previous knowledge of R is assumed.

course details



icone PDF
Programm
Course : COMPUTING-11
Duration : 2 days
Language : English
Target Audience : Cytometrists with experience in multicolor flow cytometry data analysis
Prerequisites : Good working knowledge of data analysis with standard cytometry softwares (eg.: Kaluza, FlowJo, Infinicyt)
Courses objectives : Introduction to modern computational cytometry techniques
Setting up a pipeline using the R programming language, including key steps such as data preprocessing and transformation
Automated identification of cell populations with the FlowSOM algorithm and differential analysis of sample groups.
Costs : Public Sector: 800 CHF
Industry, Pharma: 1000 CHF
Developing Countries: 500 CHF

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Private course in flow cytometry

Adapted to customer's needs

course details



Course : PRIVATE_COURSE-21
Duration : Variable
Language : English
Target Audience : Industry, Pharma, Public laboratories
Prerequisites : None
Courses objectives : From basic rules of flow cytometry to advanced applications - we can prepare a tailored course for your laboratory
Costs : available upon request

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CytHem Genève - Formation en Cytométrie Hématologique

Hématopoïèse normale, Syndromes lymphoprolifératifs, Leucémies aigües, Analyses d’évènements rares: MRD et PNH, Cytométrie spectrale, Conception de panel optimisée, Analyses multidimensionnelles (t-SNE, FlowSOM)

course details



Cours : CYTHEM-22
Durée : 22 heures (3 jours)
Langue : Français
Public cible : Hématologues et biologistes, ingénieurs, scientifiques, technicien(ne)s de laboratoire, étudiant(e)s avec un intérêt hématologie.
Prérequis : Connaissances des principes de base de la cytométrie en flux et en hématologie.
Objectifs : Programme:
• Hématopoïèse normale
• Syndromes lymphoprolifératifs B, T et NK
• Maladies plasmocytaires
• Leucémies aigües myéloïdes
• Leucémies aiguës lymphoblastiques B et T
• SMP, SMD et LMMC
• Analyses d’évènements rares: MRD et PNH
• Cytométrie spectrale
• Conception de panel optimisée
• Analyses multidimensionnelles (t-SNE, FlowSOM)
Coût : Étudiant(e)s en master ou en thèse (MD ou PhD), technicien(ne)s de laboratoire: 400 CHF
Biologistes, médecins, ingénieurs, scientifiques: 600 CHF

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Winterschool of Cell Analysis in Immunology

General review on applications of immunology analysis by flow cytometry, B-cells lymphopoiesis, development and pathology; T cell phenotypes and subsets; Assays for the assessment of T cell activation, proliferation, and function; Innate Lymphoid Cells; Myeloid lineage, myelopoiesis and normal maturation pathways; Phagocytosis, monitoring granulocyte reactivity and ROS production; Flow cytometric analysis of human monocytes and dendritic cells; Immunopathology and Sepsis, Immunoparalysis; Hypersensitivity and Basophil degranulation tests; Primary immunodeficiencies; Multicolor Panel design; QC and standardisation; ImageStream; Mass cytometry; Multi-dimensional data analysis

course details



icone PDF
Programm
Course : WINTER_SCHOOL-14
Duration : 5 days, 40 hours of lectures and practicals
Language : English
Target Audience : Immunologists, Researchers, Clinical Biologists, PhD students, Lab assistants, R&D pharma
Prerequisites : Knowledge of the basic principles of flow cytometry, strong background in immunology and/or cell biology or: Introductory course of flow cytometry
Courses objectives : The students will learn:
• to design and perform multicolor staining experiments
• to analyse in depth specific cell populations of the innate and adaptive immune system
• to perform current functional cytometric tests in immunology

Costs : Public Sector: 2000 CHF
Students (MD, PhD): 1500 CHF
Industry, Pharma: 2500 CHF
Developing Countries: 1200 CHF
Registration deadline: February 15th or when the place limit is reached.

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Certificate of Advanced Studies (CAS) in 2022/2023

Unique in Switzerland, this CAS allows the participants to acquire the necessary expertise in flow cytometry for the use of this technique in all diagnostic applications in hematology, immunology and oncology, as well as for research purposes in cellular biology, pharmacology, and microbiology. The course is composed of three modules with theoretical and practical lessons, followed by a written treatise and a written exam. Participants will obtain a “Certificate of Advanced Studies in Flow Cytometry”, issued by the University of Geneva.

course details



icone PDF
Programm
Course : CERTIFICATE-13
Duration : 1 year
Language : English
Target Audience : Physicians (hematologists, immunologists), biologists, biochemists, pharmacists, toxicologists, veterinarians, engineers in the medical sciences, laboratory technicians with a professional experience in flow cytometry.
Prerequisites : - a Master's or a Bachelor's degree in Biology, Biochemistry, Biomedical Sciences, Pharmacy, Medicine, or a degree deemed equivalent issued by a University, and recognized by the University of Geneva; - a Bachelor or a Master's degree from a University of Applied Sciences (HES). 2 years professional experience related to the Certificate program will be required. An interview can complete the admission procedure; - a higher education degree in Biomedical Analysis or equivalent training (ie.: laboratory technician). 2 years professional experience related to the Certificate program will be required. An interview can complete the admission procedure.
Courses objectives : - Give participants the theoretical and practical information needed to perform flow cytometric analyses independently
- Enable participants to develop experimental and diagnostic approaches, to develop data analysis strategies, and to use appropriate analytical softwares
- Illustrate to participants the most common applications of flow cytometry with a special focus on applications in hematology and immunology
- Inform participants of the latest developments in the field of flow cytometry, of the technical progress and of multi-dimensional data analysis
The course consists of 3 modules:
1. Introductory and refresher course in flow cytometry
2. Winter school of cell analysis in immunology
3. Flow cytometry for hematologists and immunologists
Costs : 6000.- CHF
REGISTRATION: https://www.unige.ch/formcont/cours/flow-cytometry

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Flow cytometry for biomedical analysts
Higher Professional Examination, Module Hematology

Higher Swiss Professional Examination

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Course : LABMED-08
Duration : 24 hours over 3 days (14h lectures, 10h pratical).
Language : English
Target Audience : Biomedical analysts and technicians taking part in the swiss higher education program for ”Biomedical analytics”
Prerequisites : none
Courses objectives : introduction to flow cytometry
Costs : please contact directly Miss Wyna Giller (wyna.giller@labmed.ch) for costs and registration

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MRD detection by flow cytometry

Principles and statistics of MRD detection; analysis of MRD in CLL, multiple myeloma and acute leukemias; detailed case studies; analysis of paucicellular samples.

course details



Course : MRD-07
Duration : 12 hours over 2 days (6h lectures, 6h pratical).
Language : English
Target Audience : Researchers with an interest in MRD detection, hematologists, immunologists.
Prerequisites : Knowledge of basic principles of flow cytometry, background in hematology and/or cell biology.
Courses objectives : The students will learn:
• how to perform MRD in acute leukemias and lymphomas, based on detailed studies of AML, ALL, CLL and multiple myeloma cases
• how to set up and perform analysis of paucicellular samples (ie: LCR)
Costs : Public sector: 500 CHF (up to 2 weeks prior to course start; afterwards 600 CHF).
Private sector: the fee for applicants is available upon request.

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Excellence workshop in PNH and MDS diagnosis

Flow cytometry practice (set-up, calibration and compensation), pre-analytical methods and quality control, PNH clinics and pathophysiology, detection of PNH clones in red and white blood cells, high sensitivity testing, clinical data analysis, case studies, MDS analysis by cytometry.

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Course : PNH_MDS-03
Duration : 18 hours over 3 days (6h lectures, 12h practical).
Language : English
Target Audience : Researchers with an interest in PNH, hematologists, immunologists.
Prerequisites : Knowledge of basic principles of flow cytometry; background in hematology and/or cell biology.
Courses objectives : The students will learn :
• the pathophysiology of PNH.
• how to use flow cytometry for the detection and quantification of PNH clones in white and red blood cells.
• how to perform high sensitivity analysis of very small PNH clones (<0.01%).
• the pitfalls of PNH diagnosis and how to avoid them.
Costs : Public sector: 800 CHF (up to 2 weeks prior to course start; afterwards 1000 CHF).
Private sector: the fee for applicants is available upon request.

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Private course on MRD

Refresher course on machine setup & compensation. Refresher course on sample preparation and antibody titration. MRD basic concepts and calculations. Validating tests for MRD. Quality control for MRD (UK NEQAS). Refresher course in Data analysis. MRD Case analyses: CLL, MM; ALL-B and ALL-T, AML.

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Course : SPECIAL-10
Duration : 10 Days over 2 Weeks
Language : English
Target Audience : Physicians or biologists planning to implement MRD analysis in their lab.
Prerequisites : Basic Flow Cytometry Knowledge; experience with acute leukemia diagnosis; experience with data analysis software
Courses objectives : Reviewing the basic concepts of flow cytometry and best practices of flow.

Validating the ability to detect MRD and determining the appropriate metrics.

Analysing actual cases and discussing the results and data analysis strategy.
Costs : CHF 4500.00

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