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Advanced flow cytometry for hematologists and immunologists
Multicolor panel design; harmonisation and standardisation of different flow cytometers; advanced applications in immunology (cell sorting, cell cycle analysis, apoptosis, cell proliferation); hematopoiesis and analysis of stem cells, immature, and mature cell populations; specific applications in hematology (analysis of CAR T cells, analysis of rare events, MRD analysis); advanced data analysis and detailed case studies.
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Duration : 22 hours over 3 1/2 days (10h lectures, 12h practical).
Language : English
Target Audience : Hematologists, immunologists, laboratory heads, researchers, advanced biomedical and laboratory technicians, post-docs.
Prerequisites : Knowledge of the basic principles of flow cytometry, strong background in hematology and/or cell biology.
Courses objectives : The students will learn:
• to design and perform multicolor staining experiments,
• to perform standardized set-ups with different cytometers,
• to conduct cell sorting experiments and to study cell cycle, apoptosis, and phosphorylation,
• to analyse simple case studies in hematology, including MRD.
Costs : Public sector: 900 CHF (up to 2 weeks prior to course start; afterwards 1200 CHF).
Private sector: the fee for applicants is available upon request.