Swiss Flow
Cytometry School

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Introduction to multi-dimensional flow cytometry analyses

The aim of this workshop is to introduce the participants to modern computational cytometry techniques. A mixture of theoretical and practical courses will show how to set up a pipeline using the R programming language, Cytobank, and FlowJo – including key steps such as data preprocessing and transformation, automated identification of cell populations, and differential analysis of sample groups. No previous knowledge of R is required.

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Code : 2023-COMPUTING-11
Duration : 2 days
Language : English
Target Audience : Cytometrists with experience in multicolor flow cytometry data analysis
Prerequisites : Good working knowledge of data analysis with standard cytometry software (eg.: Kaluza, FlowJo, Infinicyt)
Courses objectives : Topics:
Foundations of multidimensional data analysis.
Clustering algorithms.
Practical training on datasets in R: from pre-processing to visualization.
Clean data, clean results. Discovering trajectories in your data.
Standardization and reproducibility: the keys to generate good data for unsupervised analysis.
Accelerating answers with the Cytobank platform and autogating.
Exploring high-dimensional data in FlowJo.
Dimensionality reduction and clustering: pitfalls and practicalities.
Costs : Public Sector: 800 CHF
Industry, Pharma: 1000 CHF
Developing Countries: 500 CHF
Registration deadline: 1month before the course or when the place limit is reached.


Registration form to attend course

confirmed dates
start 2023-11-06
end 2023-11-07

COMPUTING
11

Highlighted fields are obligatory :

Birth date : Birth date :
Billing Address if different :
Do you have any specific questions or topics you would like us to address during this course ?
Please indicate why you register to this course and what are your expectations :

Pre-course evaluation form. Please help us to evaluate your level and expectations to make sure that the course adequately meets your training needs.

1. Knowledge of flow cytometry basic principles :
I have no prior knowledge of flow cytometry.
I am familiar with the basic principles of flow cytometry but I have never conducted an experiment myself.
I have run a few flow cytometry experiments under supervision but I lack the fundamental knowledge.
I am a cytometrist, please specify the number of years of experience :

2. Experience in operating a flow cytometer :
Beckman Coulter flow cytometer, please specify the model :
Becton Dickinson flow cytometer, please specify the model :
Other, please specify type and model,
or no experience :

3. Experience using analysis software :
Diva
Kaluza
Infinicyt
Other, please specify the analysis software, or none :

4. Knowledge of techniques and concepts :
Fluorescence microscopy :
None
Intermediate
Expert
 
Immunostaining :
None
Intermediate
Expert
 
Cell biology :
None
Intermediate
Expert
 
Immunology :
None
Intermediate
Expert
 
Hematology :
None
Intermediate
Expert
 

5. Please indicate special dietary requirements :

6. Do you have any comments ?
7. Confirm
The information provided will be treated in the strictest confidence in accordance with data protection legislation. Incomplete applications will not be considered. By signing this form, you confirm that the information you have given is correct and complete.
By registering I express my will to attend a course at the Swiss Flow Cytometry School in Geneva. I will take all actions needed to attend the course, including, but not limited to asking for a visa if needed, and handling my housing reservation & transport to Geneva.

I agree to pay the course fees within 30 days after having received the invoice.
Any cancellation should be done by sending an email to info@cytometryschool.ch within 2 weeks before course start.
Your registration will be confirmed by email